Agilent Technologies 1100 Series Installation Guide

Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA ©Varian, Inc. 2002 - 2006 03-914947-30:Rev.6 Agilent Technologies 1100 LC Modu

1100 LC Modules Control Manual - 10 - 03-914947-30:Rev.6 Configuring the Driver The driver software configuration is done in the Galaxie Configurati

1100 LC Modules Control Manual - 100 - 03-914947-30:Rev.6 Overview Window Click on the button: the Status Overview window is displayed and contain

1100 LC Modules Control Manual - 101 - 03-914947-30:Rev.6 General Window This window allows direct control of the autosampler. Information about t

1100 LC Modules Control Manual - 102 - 03-914947-30:Rev.6 Times Window This is a read-only window. The elapsed run time and the elapsed post run ti

1100 LC Modules Control Manual - 103 - 03-914947-30:Rev.6 Thermostat Window This is a read-only window. The actual temperature and the setpoint te

1100 LC Modules Control Manual - 104 - 03-914947-30:Rev.6 Overview Window Click on the button: the Status Overview window is displayed and contain

1100 LC Modules Control Manual - 105 - 03-914947-30:Rev.6 Overview. Click on the required sub-section to display the corresponding screen. General

1100 LC Modules Control Manual - 106 - 03-914947-30:Rev.6 Times Window This is a read-only window. The elapsed run time and the elapsed post run ti

1100 LC Modules Control Manual - 107 - 03-914947-30:Rev.6 Thermostat Window This is a read-only window. The actual temperature and the setpoint te

1100 LC Modules Control Manual - 108 - 03-914947-30:Rev.6 • Not ready: contains the not ready information sent by the instrument. • Events: contai

1100 LC Modules Control Manual - 109 - 03-914947-30:Rev.6 The button turns off all the modules that compose the system. The button turns on the

1100 LC Modules Control Manual - 11 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HP

1100 LC Modules Control Manual - 110 - 03-914947-30:Rev.6 Elution Window This is a read-only window. The actual flow rate and the pressure evolutio

1100 LC Modules Control Manual - 111 - 03-914947-30:Rev.6 Bottles Window Remaining in Bottle Parameter Group The remaining volume of the bottle is

1100 LC Modules Control Manual - 112 - 03-914947-30:Rev.6 The button turns on the pump module only. The button turns off the pump module only. •

1100 LC Modules Control Manual - 113 - 03-914947-30:Rev.6 General Window This window allows direct control of the pump. Information about the pump

1100 LC Modules Control Manual - 114 - 03-914947-30:Rev.6 By clicking on Set Flow, Set Composition, Set Flow Ramp, Turn Seal wash Off or Active Sing

1100 LC Modules Control Manual - 115 - 03-914947-30:Rev.6 The actual flow rate and the percentages of each solvent in the mobile phase are displaye

1100 LC Modules Control Manual - 116 - 03-914947-30:Rev.6 Remaining in Bottle Parameter Group The remaining volume of each bottle is indicated in th

1100 LC Modules Control Manual - 117 - 03-914947-30:Rev.6 • Not ready: contains the not ready information sent by the instrument. • Events: cont

1100 LC Modules Control Manual - 118 - 03-914947-30:Rev.6 General Window This window allows direct control of the pump. Information about the pump

1100 LC Modules Control Manual - 119 - 03-914947-30:Rev.6 The button activates the B1 channel of the Solvent Selection Valve B. The button a

1100 LC Modules Control Manual - 12 - 03-914947-30:Rev.6 NOTE: For more details about the Bus configuration, refer to the Configuring the Communica

1100 LC Modules Control Manual - 120 - 03-914947-30:Rev.6 Elution Window This is a read-only window. The actual flow rate and the percentages of ea

1100 LC Modules Control Manual - 121 - 03-914947-30:Rev.6 Bottles Window Remaining in Bottle Parameter Group The remaining volume of each bottle i

1100 LC Modules Control Manual - 122 - 03-914947-30:Rev.6 • General: displays the synopsis of the instrument, where some current values can be read

1100 LC Modules Control Manual - 123 - 03-914947-30:Rev.6 General Window This window allows direct control of the column compartments. Information

1100 LC Modules Control Manual - 124 - 03-914947-30:Rev.6 Select Set Left (or Right) temperature, then enter the desired temperature in the Left (or

1100 LC Modules Control Manual - 125 - 03-914947-30:Rev.6 The actual and setpoint temperatures for the right and left compartments are displayed V

1100 LC Modules Control Manual - 126 - 03-914947-30:Rev.6 Please note that the Injections field is automatically incremented by one after each inje

1100 LC Modules Control Manual - 127 - 03-914947-30:Rev.6 • Not ready: contains the not ready information sent by the instrument. • Events: cont

1100 LC Modules Control Manual - 128 - 03-914947-30:Rev.6 Information about the detector status is displayed: Faults, not ready parameters, etc. The

1100 LC Modules Control Manual - 129 - 03-914947-30:Rev.6 Times Window This is a read-only window. The elapsed run time and the elapsed post run t

1100 LC Modules Control Manual - 13 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 130 - 03-914947-30:Rev.6 Lamps Window This is a read-only window. The UV Lamp Ignition and On-Time are displayed.

1100 LC Modules Control Manual - 131 - 03-914947-30:Rev.6 • Not ready: contains the not ready information sent by the instrument. • Events: con

1100 LC Modules Control Manual - 132 - 03-914947-30:Rev.6 This window allows direct control of the detector. Information about the detector status i

1100 LC Modules Control Manual - 133 - 03-914947-30:Rev.6 The elapsed run time and the elapsed post run time (if any) are displayed. Signals Window

1100 LC Modules Control Manual - 134 - 03-914947-30:Rev.6 The UV and VIS Lamp Ignition and burn are displayed. When a lamp is changed, reset the cou

1100 LC Modules Control Manual - 135 - 03-914947-30:Rev.6 Status of the Agilent LC 1100 Fluorescence Detector (G1321) Click on the button to disp

1100 LC Modules Control Manual - 136 - 03-914947-30:Rev.6 The button reset the detector module. The button and button enables to take spectra.

1100 LC Modules Control Manual - 137 - 03-914947-30:Rev.6 Signal Window This is a read-only window. The current Excitation and Emission wavelength

1100 LC Modules Control Manual - 138 - 03-914947-30:Rev.6 • Not ready: contains the not ready information sent by the instrument. • Events: conta

1100 LC Modules Control Manual - 139 - 03-914947-30:Rev.6 General Window This is a read-only window. Information about the detector is displayed:

1100 LC Modules Control Manual - 14 - 03-914947-30:Rev.6 In the Plates X scrolling lists, select the type of plates installed in the Well Plate tray

1100 LC Modules Control Manual - 140 - 03-914947-30:Rev.6 The button displays the reference valve control parameters. The available valve position

1100 LC Modules Control Manual - 141 - 03-914947-30:Rev.6 Actuals Window This is a read-only window. The current Signal parameters, the current Va

1100 LC Modules Control Manual - 142 - 03-914947-30:Rev.6 The button allows displaying the recycle valve control parameters. The available valve p

1100 LC Modules Control Manual - 143 - 03-914947-30:Rev.6 Running Instrument Maintenance Software Performing Maintenance and Diagnosis Run the sep

1100 LC Modules Control Manual - 144 - 03-914947-30:Rev.6 Diagnostic Section for the Agilent Technologies LC 1100 Modules Maintenance of the Agile

1100 LC Modules Control Manual - 145 - 03-914947-30:Rev.6 The Limit values correspond to the maximum allowed values for the counters before the

1100 LC Modules Control Manual - 146 - 03-914947-30:Rev.6 Each counter modification is automatically listed in the maintenance audit trail. Press t

1100 LC Modules Control Manual - 147 - 03-914947-30:Rev.6 Info event history Limit event history Error logbook of last analysis State change histor

1100 LC Modules Control Manual - 148 - 03-914947-30:Rev.6 To finish a test, click on the button. The autosampler is then automatically re-initiali

1100 LC Modules Control Manual - 149 - 03-914947-30:Rev.6 Gripper Verification test Gripper Verification Run the Gripper Verification procedure t

1100 LC Modules Control Manual - 15 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 150 - 03-914947-30:Rev.6 Press to close the gripper and move the fingers as close as possible to the top of the v

1100 LC Modules Control Manual - 151 - 03-914947-30:Rev.6 Actions Window This window is used to list the autosampler maintenance actions. Select t

1100 LC Modules Control Manual - 152 - 03-914947-30:Rev.6 Maintenance of the Agilent LC 1100 Quaternary pump (G1311) Click on the button to displa

1100 LC Modules Control Manual - 153 - 03-914947-30:Rev.6 Select the MCGV in the module schema. The following counters limits, actual and accumula

1100 LC Modules Control Manual - 154 - 03-914947-30:Rev.6 The Limit values correspond to the maximum allowed values for the counters before the mai

1100 LC Modules Control Manual - 155 - 03-914947-30:Rev.6 Each counter modification is automatically listed in the maintenance audit trail. Press

1100 LC Modules Control Manual - 156 - 03-914947-30:Rev.6 MCGV switches A: Total number of channel A valve switches. MCGV switches B: Total number o

1100 LC Modules Control Manual - 157 - 03-914947-30:Rev.6 Limit event history of last analysis Parameters changes during the last analysis Run hist

1100 LC Modules Control Manual - 158 - 03-914947-30:Rev.6 Perform the Pressure Test to demonstrate pressure–tightness up to 400 bars. The test cons

1100 LC Modules Control Manual - 159 - 03-914947-30:Rev.6 For analyzing the test results, refer to the pump operating manual. Leak test Run the Le

1100 LC Modules Control Manual - 16 - 03-914947-30:Rev.6 For the G1312 module, the following screen is displayed:

1100 LC Modules Control Manual - 160 - 03-914947-30:Rev.6 Leak test pressure profile05010015020025030035040001234567Time (min)Pressure (bar) Ramp 1:

1100 LC Modules Control Manual - 161 - 03-914947-30:Rev.6 For analyzing the test results, refer to the pump operating manual.

1100 LC Modules Control Manual - 162 - 03-914947-30:Rev.6 Actions Window This window is used to list the pump module maintenance actions. Select th

1100 LC Modules Control Manual - 163 - 03-914947-30:Rev.6 Pump pistons replaced: no outcome on counters. Purge valve frit replaced: no outcome on c

1100 LC Modules Control Manual - 164 - 03-914947-30:Rev.6 The Limit values correspond to the maximum allowed values for the counters before the ma

1100 LC Modules Control Manual - 165 - 03-914947-30:Rev.6 Each counter modification is automatically listed in the maintenance audit trail. Press

1100 LC Modules Control Manual - 166 - 03-914947-30:Rev.6 Logbook Window Select in the scrolling list the logbook to display. The available logbook

1100 LC Modules Control Manual - 167 - 03-914947-30:Rev.6 Tests Window Select a test in the scrolling list and press to start the test. The avail

1100 LC Modules Control Manual - 168 - 03-914947-30:Rev.6 Self-Test Test The Self-Test test consists in running the following individual tests con

1100 LC Modules Control Manual - 169 - 03-914947-30:Rev.6 For analyzing the test results, refer to the detector operating manual. Dark Current Tes

1100 LC Modules Control Manual - 17 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 170 - 03-914947-30:Rev.6 For analyzing the test results, refer to the detector operating manual. Intensity Test Pe

1100 LC Modules Control Manual - 171 - 03-914947-30:Rev.6 For analyzing the test results, refer to the detector operating manual. Holmium Test Per

1100 LC Modules Control Manual - 172 - 03-914947-30:Rev.6 For analyzing the test results, refer to the detector operating manual. Cell Test Perfor

1100 LC Modules Control Manual - 173 - 03-914947-30:Rev.6 For analyzing the test results, refer to the detector operating manual. Filter Test Perf

1100 LC Modules Control Manual - 174 - 03-914947-30:Rev.6 For analyzing the test results, refer to the detector operating manual. Wavelength Calibra

1100 LC Modules Control Manual - 175 - 03-914947-30:Rev.6 During the test, a theoretical analog signal of approximately 52 mV with a square wave of

1100 LC Modules Control Manual - 176 - 03-914947-30:Rev.6 Actions Window This window is used to list the detector module maintenance actions. Selec

1100 LC Modules Control Manual - 177 - 03-914947-30:Rev.6 UV lamp replaced: UV lamp ignition, UV lamp Burn and UV lamp On Time counters can be rese

1100 LC Modules Control Manual - 178 - 03-914947-30:Rev.6 Appendix GPIB Addresses The 1100 Master module connected to Galaxie with the GPIB cable

1100 LC Modules Control Manual - 179 - 03-914947-30:Rev.6 10 Down Up Down Up Down 11 Down Up Down Up Up 12 Down Up Up Down Down 13 Down Up

1100 LC Modules Control Manual - 18 - 03-914947-30:Rev.6 For the G1311 module, the following screen is displayed:

1100 LC Modules Control Manual - 180 - 03-914947-30:Rev.6 Configuring a Fixed IP Address This appendix describes how to fix the IP address in the A

1100 LC Modules Control Manual - 181 - 03-914947-30:Rev.6 3. Click on the button on the left of the screen. 4. Click on the Configuration tab.

1100 LC Modules Control Manual - 182 - 03-914947-30:Rev.6 5. Select Manual in the TCP Configuration Type dropdown list.

1100 LC Modules Control Manual - 183 - 03-914947-30:Rev.6 6. Type the desired IP address, subnet mask and Gateway in the corresponding fields. 7

1100 LC Modules Control Manual - 184 - 03-914947-30:Rev.6 8. Reboot the HPLC chain.

1100 LC Modules Control Manual - 19 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 2 - 03-914947-30:Rev.6 Table of Contents Introduction...

1100 LC Modules Control Manual - 20 - 03-914947-30:Rev.6 Configuring the Agilent Technologies LC 1100 Column Compartment Click on the button to di

1100 LC Modules Control Manual - 21 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 22 - 03-914947-30:Rev.6 Configuring the Agilent Technologies LC 1100 Detectors The Agilent Technologies 1100 Variab

1100 LC Modules Control Manual - 23 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 24 - 03-914947-30:Rev.6 Miscellaneous Parameter Group Check or uncheck the Galaxie run started by this module (No c

1100 LC Modules Control Manual - 25 - 03-914947-30:Rev.6 Communications Parameter Group Master module HPIB Address: Select this option if the HPLC

1100 LC Modules Control Manual - 26 - 03-914947-30:Rev.6 Configuring the Synopsis In the Overview part of the system configuration, a synopsis can b

1100 LC Modules Control Manual - 27 - 03-914947-30:Rev.6 (temperature, flow, signal, etc.) depending on the installed devices. For example, the fol

1100 LC Modules Control Manual - 28 - 03-914947-30:Rev.6 Agilent 1100 detectors Galaxie Configuration Manager Screen Agilent 1100 detectors Galaxi

1100 LC Modules Control Manual - 29 - 03-914947-30:Rev.6 The method created on this system will have the following structure: However, if some of

1100 LC Modules Control Manual - 3 - 03-914947-30:Rev.6 Status of the Agilent LC 1100 Binary Pump (G1312)...

1100 LC Modules Control Manual - 30 - 03-914947-30:Rev.6 The icon indicates that the corresponding channel is activated. The icon indicates that

1100 LC Modules Control Manual - 31 - 03-914947-30:Rev.6 Building a Method Creating a New Method The method contains parameters for instrument cont

1100 LC Modules Control Manual - 32 - 03-914947-30:Rev.6 2. Choose the system (chromatograph) associated with the method. A method is created for

1100 LC Modules Control Manual - 33 - 03-914947-30:Rev.6 4. Enter the New Method Name in the first field. In the Description field, enter informa

1100 LC Modules Control Manual - 34 - 03-914947-30:Rev.6 Control Method Section for the Agilent Technologies LC 1100 Modules Once the new method is

1100 LC Modules Control Manual - 35 - 03-914947-30:Rev.6 Press the button to display the System Control Method Advanced Tools window: to send t

1100 LC Modules Control Manual - 36 - 03-914947-30:Rev.6 Control Section for the Agilent LC 1100 AutoSampler (G1313) Click on the button to displa

1100 LC Modules Control Manual - 37 - 03-914947-30:Rev.6 Others Parameter Group In the Needle Offset field, enter the vertical needle offset value.

1100 LC Modules Control Manual - 38 - 03-914947-30:Rev.6 • Injection user program: when selecting this injection mode, another tab is displayed all

1100 LC Modules Control Manual - 39 - 03-914947-30:Rev.6 After a new line has been added, double-click on it to edit. A new window is displayed: U

1100 LC Modules Control Manual - 4 - 03-914947-30:Rev.6 Introduction General This document describes the configuration of the Agilent Technologies

1100 LC Modules Control Manual - 40 - 03-914947-30:Rev.6 Move Syringe back and forth Move Syringe home Move Syringe service Move vial to new locatio

1100 LC Modules Control Manual - 41 - 03-914947-30:Rev.6 Speeds Parameter Group In the Sample Drawing Speed field, enter the sample aspiration spe

1100 LC Modules Control Manual - 42 - 03-914947-30:Rev.6 • Injection user program: when selecting this injection mode, another tab is displayed

1100 LC Modules Control Manual - 43 - 03-914947-30:Rev.6 Use the following buttons to edit the table: to add a line to the table. to delete a

1100 LC Modules Control Manual - 44 - 03-914947-30:Rev.6 Use the scrolling list to choose the action to program. The available actions are: Draw fr

1100 LC Modules Control Manual - 45 - 03-914947-30:Rev.6 Depending on the selected action, the parameters to fill in will be different. Thermost

1100 LC Modules Control Manual - 46 - 03-914947-30:Rev.6 Speeds Parameter Group In the Sample Drawing Speed field, enter the sample aspiration spee

1100 LC Modules Control Manual - 47 - 03-914947-30:Rev.6 Others Parameter Group In the Needle Offset field, enter the vertical needle offset value.

1100 LC Modules Control Manual - 48 - 03-914947-30:Rev.6 • Injection user program: when selecting this injection mode, another tab is displayed a

1100 LC Modules Control Manual - 49 - 03-914947-30:Rev.6 Use the following buttons to edit the table: to add a line to the table. to delete a

1100 LC Modules Control Manual - 5 - 03-914947-30:Rev.6 Installation and Configuration Installing the Driver Software The Galaxie software must al

1100 LC Modules Control Manual - 50 - 03-914947-30:Rev.6 Use the scrolling list to choose the action to program. The available actions are: Draw fr

1100 LC Modules Control Manual - 51 - 03-914947-30:Rev.6 Control Section for the Agilent LC 1100 Isocratic Pump (G1310) Click on the button to di

1100 LC Modules Control Manual - 52 - 03-914947-30:Rev.6 Settings Control Prerun Elution Parameter Group In the Flow field, enter the total flow ra

1100 LC Modules Control Manual - 53 - 03-914947-30:Rev.6 Use the following buttons to edit the table: to add a line to the table. to delete a lin

1100 LC Modules Control Manual - 54 - 03-914947-30:Rev.6 Pressure Alarms Parameter Group Enter the Minimum and Maximum pressure limits of the pump.

1100 LC Modules Control Manual - 55 - 03-914947-30:Rev.6 Compressibility Parameter Group To take into account the compressibility of the solvents,

1100 LC Modules Control Manual - 56 - 03-914947-30:Rev.6 General Control Miscellaneous Parameter Group Check Automatic Pump Stop to turn off the pu

1100 LC Modules Control Manual - 57 - 03-914947-30:Rev.6 Settings Control Prerun Elution Parameter Group In the Flow field, enter the total flow r

1100 LC Modules Control Manual - 58 - 03-914947-30:Rev.6 composition. The time is the elapsed time since the beginning of the run. The Pump Gradient

1100 LC Modules Control Manual - 59 - 03-914947-30:Rev.6 Pressure Alarms Parameter Group Enter the Minimum and Maximum pressure limits of the pump.

1100 LC Modules Control Manual - 6 - 03-914947-30:Rev.6 NOTE: For more details about the configuration of a GPIB_Interface or an NI488 bus, refer t

1100 LC Modules Control Manual - 60 - 03-914947-30:Rev.6 Compressibility Parameter Group To take into account the compressibility of the solvents, s

1100 LC Modules Control Manual - 61 - 03-914947-30:Rev.6 General Control Check Automatic Pump Stop to turn off the pump at the end of a sequence

1100 LC Modules Control Manual - 62 - 03-914947-30:Rev.6 Settings Control Prerun Elution Parameter Group In the Flow field, enter the total flow ra

1100 LC Modules Control Manual - 63 - 03-914947-30:Rev.6 Program Parameter Group Program an elution flow and/or a composition gradient. Each step o

1100 LC Modules Control Manual - 64 - 03-914947-30:Rev.6 Limits Control Pressure Alarms Parameter Group Enter the Minimum and Maximum pressure limi

1100 LC Modules Control Manual - 65 - 03-914947-30:Rev.6 Control Control Left and Right Drive Compressibility Parameter Group For each drive, to t

1100 LC Modules Control Manual - 66 - 03-914947-30:Rev.6 Control Section for the Agilent LC 1100 Column Compartment (G1316) Click on the button to

1100 LC Modules Control Manual - 67 - 03-914947-30:Rev.6 Left Compartment sub-section (the Right Compartment sub-section will not be active). Check

1100 LC Modules Control Manual - 68 - 03-914947-30:Rev.6 Left Compartment Prerun Setpoints Parameter Group Check the On box to activate the column c

1100 LC Modules Control Manual - 69 - 03-914947-30:Rev.6 Valves Control Valve Prerun Position Parameter Group Select the position of the valve bef

1100 LC Modules Control Manual - 7 - 03-914947-30:Rev.6 Ethernet Communications The Agilent Technologies 1100 modules can use Ethernet ® as the met

1100 LC Modules Control Manual - 70 - 03-914947-30:Rev.6 Valve Program Parameter Group Program valve position changes during the run. The time is th

1100 LC Modules Control Manual - 71 - 03-914947-30:Rev.6 Settings Control Signal Parameter Group In the Wavelength field, enter the value of the i

1100 LC Modules Control Manual - 72 - 03-914947-30:Rev.6 <0.005 min (RiseTime<0.12 sec) (DataRate=13.74 Hz) >0.005 min (RiseTime>0.12 se

1100 LC Modules Control Manual - 73 - 03-914947-30:Rev.6 In the Range scrolling list, enter the output voltage range value. The available values ar

1100 LC Modules Control Manual - 74 - 03-914947-30:Rev.6 Wavelength Parameter Group In the Wavelength Start field, enter the minimum spectra wavele

1100 LC Modules Control Manual - 75 - 03-914947-30:Rev.6 Time Table Control Program Parameter Group Program detector changes during the run. In t

1100 LC Modules Control Manual - 76 - 03-914947-30:Rev.6 to delete a line in the table. to clear the entire table. Control Section for the Agilent

1100 LC Modules Control Manual - 77 - 03-914947-30:Rev.6 Signals Control Sample Diode Parameter Group Define the center wavelength in the Wl field

1100 LC Modules Control Manual - 78 - 03-914947-30:Rev.6 Define the center wavelength in the Wl field and the bandwidth in the Bw field. The signal

1100 LC Modules Control Manual - 79 - 03-914947-30:Rev.6 >0.85 min (Response Time =16 sec) (DataRate=0.31 Hz) Analogs Control This detector ha

1100 LC Modules Control Manual - 8 - 03-914947-30:Rev.6 GPIB cable81-839700-00GPIB boardGalaxie Acquisition ServerLANGPIB cable Extender81-839704-00

1100 LC Modules Control Manual - 80 - 03-914947-30:Rev.6 Spectra Control Wavelength Parameter Group In the Wavelength Start field, enter the minimu

1100 LC Modules Control Manual - 81 - 03-914947-30:Rev.6 Peak Detector Parameter Group In the Peak Detector Threshold field, enter the peak detecto

1100 LC Modules Control Manual - 82 - 03-914947-30:Rev.6 to add a line to the table. to delete a line in the table. to clear the entire table. C

1100 LC Modules Control Manual - 83 - 03-914947-30:Rev.6 Settings Control Mode Parameter Group Choose the Acquisition Mode between Fluorescence, P

1100 LC Modules Control Manual - 84 - 03-914947-30:Rev.6 Excitation Parameter Group Check Wavelength A to Wavelength D to activate the corresponding

1100 LC Modules Control Manual - 85 - 03-914947-30:Rev.6 Time Table Control Program Parameter Group Program detector changes during the run. Chan

1100 LC Modules Control Manual - 86 - 03-914947-30:Rev.6 to clear the entire table. Baseline Behavior Parameter Group Select in the scrolling list

1100 LC Modules Control Manual - 87 - 03-914947-30:Rev.6 In the Attenuation scrolling list, select the attenuation value. The available values are:

1100 LC Modules Control Manual - 88 - 03-914947-30:Rev.6 Settings Parameter Group Enter the Delay in the corresponding field. Enter the Gate in the

1100 LC Modules Control Manual - 89 - 03-914947-30:Rev.6 Photo Multiplier Tube Parameter Group Select the value of the Gain parameter (amplificatio

1100 LC Modules Control Manual - 9 - 03-914947-30:Rev.6 Galaxie Acquisition ServerGPIB cable81-839700-00National Instrument GPIB boardCAN Cablespro

1100 LC Modules Control Manual - 90 - 03-914947-30:Rev.6 Settings Control General Parameter Group Check Heater On to turn the heater on. In the Tem

1100 LC Modules Control Manual - 91 - 03-914947-30:Rev.6 Automatic Purge Parameter Group In the Purge time field, enter the time during which the i

1100 LC Modules Control Manual - 92 - 03-914947-30:Rev.6 Analogs Control In the Offset field, enter the offset value to adjust the baseline. In the

1100 LC Modules Control Manual - 93 - 03-914947-30:Rev.6 Time Table Control Program Parameter Group Program peakwidth and/or the polarity changes

1100 LC Modules Control Manual - 94 - 03-914947-30:Rev.6 Starting and Viewing an Acquisition Quick Start and Sequence Once the instrument control m

1100 LC Modules Control Manual - 95 - 03-914947-30:Rev.6 In the Acquisition parameters tab, required fields like the Run Name, the Run ID and the

1100 LC Modules Control Manual - 96 - 03-914947-30:Rev.6 Sequence 1. To create a sequence, select the FILE / NEW SEQUENCE menu, and complete the di

1100 LC Modules Control Manual - 97 - 03-914947-30:Rev.6 Running Acquisition Status Viewing a Running Acquisition To view a running acquisition, cl

1100 LC Modules Control Manual - 98 - 03-914947-30:Rev.6 The button turns on all the modules that compose the system. The button turns off all th

1100 LC Modules Control Manual - 99 - 03-914947-30:Rev.6 Injection Window This is a read-only window. The vial and the injection volume are displa