Agilent Technologies E5500 Series User's Guide Page 1
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- Table of contents
- TROUBLESHOOTING
- BOOKMARKS
- AB Library Builder 1
- Contents 3
- About This Guide 7
- About the Products 9
- How to use the 10
- Kit contents 11
- System: 5500 Series SOLiD 12
- Systems User Guide 12
- Chapter1 About the Products 12
- Workflow 13
- Procedural guidelines 13
- Shear the DNA 14
- Quantitate the DNA 14
- 5. Remove the sheared DNA: 16
- Set up the AB Library Builder 17
- System for size-selected or 17
- Insert or change 18
- Load the racks and 19
- Load the cartridge rack 20
- Insert the racks 23
- Insert the tip and tube rack 25
- Build the library 27
- Set up for a new run 28
- Optional Amplification 29
- Purify the nick 30
- Prepare the 31
- Troubleshooting 35
- Chapter5 Troubleshooting 36
- Instrument error codes 38
- Ordering Information 39
- Required equipment 42
- Optional equipment 44
- Replacement parts 44
- Required consumables 45
- Optional consumables 46
- Supplemental Procedures 47
- System operation 48
- Manage the run 50
- Size-select the 51
- 250-bp marker 54
- Collection wells 54
- Sample wells 54
- ND-1000 55
- Spectrophotometer 55
- 2. Open the NanoDrop 56
- Supplemental Background 59
- Information 59
- Genomic DNA Sheared DNA 60
- Library molecule ligated 60
- Sequencers User 62
- Guide (Part no. 4456991) 62
- Library Construction 63
- Oligonucleotide Sequences 63
- System Kit 73
- Safety 77
- Chemical waste safety 78
- Chemical waste 79
- Waste disposal 79
- Biological hazard safety 80
- Documentation and Support 81
- Glossary 83
- P1-T Adaptor 84
- Standard Adaptor 84
- 4460965A 87
- Headquarters 87
Summary of Contents
USER GUIDEFragment Library Preparation Using theAB Library Builder™ System5500 Series SOLiD™ SystemsPublication Part Number 4460965 Rev. ARevision Dat
10Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter1 About the ProductsProduct inform
11Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter1 About the ProductsKit contents a
12Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter1 About the ProductsKit contents a
13Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER2 Prepare to Build the LibraryWor
14Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibraryQuan
15Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibraryShea
16Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibraryShea
17Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
18Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
19Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.This user guide is the proprietary material of Applied Biosy
20Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
21Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
22Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
23Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
24Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
25Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
26Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet
27Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER3 Build the libraryWorkflowFor ad
28Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter3 Build the librarySet up for a ne
29Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER4 Nick-Translate the Library with
Contents3Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide About This Guide . . . . . . . .
30Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with
31Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with
32Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with
33Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with
34Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with
35Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER5 TroubleshootingFor symptoms oth
36Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter5 TroubleshootingRun stops after a
37Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter5 Troubleshooting After the automa
38Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter5 TroubleshootingInstrument error
39Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAAPPENDIXA Ordering InformationThis appe
4Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideContentsAPPENDIX A Ordering Information .
40Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired Ap
41Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired Ap
42Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired eq
43Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired eq
44Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationOptional eq
45Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired co
46Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationOptional co
47Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideBAPPENDIXB Supplemental ProceduresThis a
48Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresAB Libra
49Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresAB Libra
5Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideContents Glossary . . . . . . . . . . . . .
50Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresAB Libra
51Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona
52Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona
53Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona
54Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona
55Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita
56Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita
57Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita
58Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita
59Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCAPPENDIXC Supplemental BackgroundInform
6Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideContents
60Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixC Supplemental Background Informa
61Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixC Supplemental Background Informa
62Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixC Supplemental Background Informa
63Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideDAPPENDIXD Library ConstructionOligonucl
64Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
65Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
66Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
67Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
68Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
69Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
7Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide About This GuideSafety informationNote: For
70Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
71Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
72Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle
73Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideEAPPENDIXE Checklist and workflow tracki
74Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixE Checklist and workflow tracking
75Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixE Checklist and workflow tracking
76Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixE Checklist and workflow tracking
77Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideFAPPENDIXF SafetyThis appendix covers:
78Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixF SafetySDSsFChemical safety guid
79Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixF SafetyChemical waste safetyF WA
8Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAbout This GuideSafety informationSDSs The S
80Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixF SafetyBiological hazard safetyF
81Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide Documentation and SupportRelated documenta
82Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideDocumentation and SupportObtaining support
83Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide Glossarybarcode A short, unique sequence t
84Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideGlossaryP1-T AdaptorA T-tailed double-stran
85Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide IndexAAB Library Builder Systemrunning 27A
86Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideIndexNNanoDrop® ND-1000 Spectrophotometer 5
4460965AHeadquarters5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1 760 603 7200 | Toll Free in USA 800 955 6288For support visit www.app
9Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER1 About the ProductsNote: For comp
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