Agilent Technologies E5500 Series User's Guide download pdf (Page 55)

Fragment Library Preparation Using the AB Library Builder

™

System: 5500 Series SOLiD

™

Systems User Guide

AppendixB Supplemental Procedures

Quantitate the DNA with the NanoDrop

ND-1000 Spectrophotometer

Quantitate the DNA with the NanoDrop

ND-1000

Spectrophotometer

The Thermo Scientific NanoDrop

1000 Spectrophotometer measures nucleic acid

samples from 2 ng/µL–3700 ng/µL without dilution.

Materials and

equipment

required

Procedure

1. Ensure that the NanoDrop

ND-1000 Spectrophotometer is properly calibrated.

Use the CF-1 Calibration Fluid Kit if necessary.

Required equipment

Item

†

† Applied Biosystems has validated this protocol using this specific material. Substitution may adversely affect

system performance.

Source

NanoDrop

ND-1000 Spectrophotometer (computer required) Thermo Scientific

ND-1000

Pipettors (20 µL) Major Laboratory

Supplier (MLS)

‡

‡ For the SDS of any chemical not distributed by Applied Biosystems, contact the chemical manufacturer.

Before handling any chemicals, refer to the SDS provided by the manufacturer, and observe all relevant

precautions.

Required consumables

Item

†

† Applied Biosystems has validated this protocol using this specific material. Substitution may adversely affect

system performance.

Source

Nuclease-free Water (1 L) Applied Biosystems

AM9932

CF-1 Calibration Fluid Kit

‡

‡The NanoDrop

Conditioning Kit is useful for “reconditioning” the sample measurement pedestals to a

hydrophobic state if they become “unconditioned.” (Refer to the NanoDrop

Conditioning Kit user's manual

for more information.) The PR-1 kit consists of a container of specially formulated polishing compound and

a supply of convenient applicators.

Thermo Scientific

CF-1

PR Conditioning Kit Thermo Scientific

PR-1

Filtered pipettor tips Major Laboratory

Supplier (MLS)

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Agilent Technologies E5500 Series User's Guide Page 55

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