Agilent Technologies Agilent 35900E Service Manual

Agilent TechnologiesAgilent ChemStationUnderstanding Your ChemStationUnderstanding Your Agilent ChemS tation

10 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesIn This Guide...Navigation Buttons 38ChemStation Explorer 38This chapter descri

100 Understanding Your Agilent ChemStation4 IntegrationBaseline AllocationB The peak started or stopped on the baseline.V The peak started or stopped

Understanding Your Agilent ChemStation 101Integration4Baseline Allocationf Peak defined by a front shoulder tangent.b Peak defined by a rear shoulder

102 Understanding Your Agilent ChemStation4 IntegrationPeak Area MeasurementPeak Area MeasurementThe final step in peak integration is determining the

Understanding Your Agilent ChemStation 103Integration4Peak Area Measurement• for valley-to-valley (VV) peaks, the area above the baseline, segmented w

104 Understanding Your Agilent ChemStation4 IntegrationPeak Area MeasurementFigure 26 Area Measurement for Negative PeaksUnits and Conversion FactorsE

Understanding Your Agilent ChemStation 105Integration4Integration EventsIntegration EventsThe integrator provides you with a number of initial and tim

106 Understanding Your Agilent ChemStation4 IntegrationIntegration EventsChoosing Peak WidthChoose the setting that provides just enough filtering to

Understanding Your Agilent ChemStation 107Integration4Integration EventsTuning IntegrationIt is often useful to change the values for the slope sensit

108 Understanding Your Agilent ChemStation4 IntegrationIntegration EventsTimed EventsYou can use timed events to customize signal baseline constructio

Understanding Your Agilent ChemStation 109Integration4Integration Events0.2% for GC. The initial area reject is set to zero and a trial integration is

Understanding Your Agilent ChemStation 11Agilent ChemStation Features1General DescriptionGeneral DescriptionThe ChemStations for GC, LC, LC/MSD, CE, C

110 Understanding Your Agilent ChemStation4 IntegrationManual IntegrationManual Integration This type of integration allows you to integrate selected

Understanding Your Agilent ChemStation 111Integration4Manual IntegrationSaving Manual Integration EventsAs manual integration events are typically ver

112 Understanding Your Agilent ChemStation4 IntegrationManual IntegrationIn case the Manual Events checkbox of the Integration Events Table of a metho

113Understanding Your Agilent ChemStationAgilent Technologies5QuantificationWhat is Quantification? 114Quantification Calculations 115Correction Facto

114 Understanding Your Agilent ChemStation5 QuantificationWhat is Quantification?What is Quantification?After the peaks have been integrated and ident

Understanding Your Agilent ChemStation 115Quantification5Quantification CalculationsQuantification Calculations The ChemStation offers the following c

116 Understanding Your Agilent ChemStation5 QuantificationCorrection FactorsCorrection Factors The quantification calculations use four correction fac

Understanding Your Agilent ChemStation 117Quantification5Correction FactorsSample Amount If the ESTD% or ISTD% calculations are selected, the ESTD and

118 Understanding Your Agilent ChemStation5 QuantificationUncalibrated Calculation ProceduresUncalibrated Calculation Procedures Uncalibrated calculat

Understanding Your Agilent ChemStation 119Quantification5Calibrated Calculation ProceduresCalibrated Calculation Procedures The external standard (EST

12 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesGeneral Description• a single instrument analog-to-digital (A/D) ChemStation fo

120 Understanding Your Agilent ChemStation5 QuantificationESTD CalculationESTD Calculation The ESTD procedure is the basic quantification procedure in

Understanding Your Agilent ChemStation 121Quantification5ESTD CalculationD is the dilution factor.Figure 28 Response FactorThe multiplier and dilution

122 Understanding Your Agilent ChemStation5 QuantificationNorm% CalculationNorm% Calculation In the normalization method, response factors are applied

Understanding Your Agilent ChemStation 123Quantification5ISTD CalculationISTD Calculation The ISTD procedure eliminates the disadvantages of the ESTD

124 Understanding Your Agilent ChemStation5 QuantificationISTD CalculationRun 1: Calibration1 The calibration points are constructed by calculating an

Understanding Your Agilent ChemStation 125Quantification5ISTD CalculationISTD Calculation of Calibrated PeaksThe equations used to calculate the actua

126 Understanding Your Agilent ChemStation5 QuantificationISTD CalculationRFx is the Response Factor set in the Calibration Settings dialog box.You ca

127Understanding Your Agilent ChemStationAgilent Technologies6Peak IdentificationWhat is Peak Identification? 128Peak Matching Rules 129Types of Peak

128 Understanding Your Agilent ChemStation6 Peak IdentificationWhat is Peak Identification?What is Peak Identification? Peak identification identifies

Understanding Your Agilent ChemStation 129Peak Identification6Peak Matching RulesPeak Matching Rules The following rules apply to the peak matching pr

Understanding Your Agilent ChemStation 13Agilent ChemStation Features1General Descriptionconfigured, no more than two diode array detectors are suppor

130 Understanding Your Agilent ChemStation6 Peak IdentificationTypes of Peak IdentificationTypes of Peak Identification There are different techniques

Understanding Your Agilent ChemStation 131Peak Identification6Types of Peak IdentificationAmount Limits The amount limits defined in the Compound Deta

132 Understanding Your Agilent ChemStation6 Peak IdentificationAbsolute Retention/Migration TimeAbsolute Retention/Migration Time A retention/migratio

Understanding Your Agilent ChemStation 133Peak Identification6Absolute Retention/Migration Time• specific window values for individual components whic

134 Understanding Your Agilent ChemStation6 Peak IdentificationCorrected Retention/Migration TimesCorrected Retention/Migration Times To match peaks b

Understanding Your Agilent ChemStation 135Peak Identification6Corrected Retention/Migration Timesprogresses. Often during a long run the retention/mig

136 Understanding Your Agilent ChemStation6 Peak IdentificationPeak QualifiersPeak Qualifiers A component can be detected with more than one signal. A

Understanding Your Agilent ChemStation 137Peak Identification6Peak QualifiersSignal CorrelationSignal correlation means that two peaks measured in dif

138 Understanding Your Agilent ChemStation6 Peak IdentificationPeak QualifiersThe tolerance can be set in the calibration table user interface (Identi

Understanding Your Agilent ChemStation 139Peak Identification6The Identification ProcessThe Identification Process When attempting to identify peaks,

14 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesChemStation HardwareChemStation HardwareFor details of ChemStation hardware, se

140 Understanding Your Agilent ChemStation6 Peak IdentificationThe Identification ProcessFinding the Remaining Calibrated PeaksThe third pass identifi

141Understanding Your Agilent ChemStationAgilent Technologies7CalibrationDefinition of Terms 142Calibration Table 143Calibration Curve 144Unknown Samp

142 Understanding Your Agilent ChemStation7 CalibrationDefinition of TermsDefinition of Terms Calibration Calibration is the process of determining re

Understanding Your Agilent ChemStation 143Calibration7Calibration TableCalibration Table The calibration table specifies conversions of peak areas or

144 Understanding Your Agilent ChemStation7 CalibrationCalibration CurveCalibration Curve A calibration curve is a graphical presentation of the amoun

Understanding Your Agilent ChemStation 145Calibration7Calibration Curvewhere:relRES = relative residual in percentThe calculated response represents t

146 Understanding Your Agilent ChemStation7 CalibrationUnknown SamplesUnknown Samples An unknown sample is a sample containing an unknown amount of th

Understanding Your Agilent ChemStation 147Calibration7Types of CalibrationTypes of Calibration The ChemStation offers two types of calibrations, singl

148 Understanding Your Agilent ChemStation7 CalibrationTypes of CalibrationFigure 35 Two-level calibration curveFor example, if you want to quantify a

Understanding Your Agilent ChemStation 149Calibration7Types of Calibrationassumed to be linear. The difference between the two calibration types is th

Understanding Your Agilent ChemStation 15Agilent ChemStation Features1About the ChemStation SoftwareAbout the ChemStation SoftwareOperating SystemThe

150 Understanding Your Agilent ChemStation7 CalibrationTypes of CalibrationCalibration RangesEach multilevel calibration is valid over the range of co

Understanding Your Agilent ChemStation 151Calibration7Types of CalibrationTo force the origin to be included in the calibration curve the calibration

152 Understanding Your Agilent ChemStation7 CalibrationTypes of CalibrationQuadratic calibration point weightings, for example, can be used to adjust

Understanding Your Agilent ChemStation 153Calibration7Group CalibrationGroup CalibrationGroup calibration can be applied for compounds where the indiv

154 Understanding Your Agilent ChemStation7 CalibrationPeak SummingPeak SummingThe peak sum table is provided for certain applications in the petroche

Understanding Your Agilent ChemStation 155Calibration7RecalibrationRecalibrationWhat is Recalibration?Recalibration is the process used when you want

156 Understanding Your Agilent ChemStation7 CalibrationRecalibrationwhere the same compounds have been analyzed for many years and the response factor

Understanding Your Agilent ChemStation 157Calibration7RecalibrationWays to Recalibrate Recalibration can be done in two ways using the ChemStation sof

158 Understanding Your Agilent ChemStation7 CalibrationRecalibration

159Understanding Your Agilent ChemStationAgilent Technologies8AutomationWhat is Automation? 161What is a Sequence/Sequence Template? 162Preferences -

16 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareData ModelThe ChemStation software is designed ar

160 Understanding Your Agilent ChemStation8 AutomationRecalibrationSpecifying Recalibrations 180Recalibration Parameters in the Sequence Table 180Type

Understanding Your Agilent ChemStation 161Automation8What is Automation?What is Automation? Automation is the unattended analysis of more than one inj

162 Understanding Your Agilent ChemStation8 AutomationWhat is a Sequence/Sequence Template?What is a Sequence/Sequence Template? A sequence is a serie

Understanding Your Agilent ChemStation 163Automation8Preferences - Sequence TabPreferences - Sequence TabOn the Sequence tab, the user has the choice

164 Understanding Your Agilent ChemStation8 AutomationPreferences - Sequence TabUnique Folder Creation OFFIn this mode of data storage, the method nam

Understanding Your Agilent ChemStation 165Automation8Sequence ParametersSequence Parameters The Sequence Parameters dialog box contains information co

166 Understanding Your Agilent ChemStation8 AutomationSequence TableSequence Table The sequence table determines which methods are used to analyze the

Understanding Your Agilent ChemStation 167Automation8Creating Sequences (Sequences and Sequence Templates)Creating Sequences (Sequences and Sequence T

168 Understanding Your Agilent ChemStation8 AutomationCreating Sequences (Sequences and Sequence Templates)Using the Custom Fields ButtonIf custom fie

Understanding Your Agilent ChemStation 169Automation8Working with Sequences (Sequences and Sequence Templates)Working with Sequences (Sequences and Se

Understanding Your Agilent ChemStation 17Agilent ChemStation Features1About the ChemStation Software• COMx (where x is a number from 1 to 9)• LPT1x (w

170 Understanding Your Agilent ChemStation8 AutomationWorking with Sequences (Sequences and Sequence Templates)Aborting a Sequence The abort function

Understanding Your Agilent ChemStation 171Automation8Working with Sequences (Sequences and Sequence Templates)Figure 38 Partial Sequence Dialog BoxPar

172 Understanding Your Agilent ChemStation8 AutomationSequence Log FileSequence Log File A sequence log file is produced that indicates what has happe

Understanding Your Agilent ChemStation 173Automation8What Happens When a Sequence is Run?What Happens When a Sequence is Run? Starting a Sequence usin

174 Understanding Your Agilent ChemStation8 AutomationWhat Happens When a Sequence is Run?• During the entire process, the ChemStation tracks the sequ

Understanding Your Agilent ChemStation 175Automation8Sequence Data File Structure (Unique Folder Creation ON)Sequence Data File Structure (Unique Fold

176 Understanding Your Agilent ChemStation8 AutomationData File Naming in a SequenceData File Naming in a Sequence Data file naming in a sequence can

Understanding Your Agilent ChemStation 177Automation8Data File Naming in a SequenceEntering Data File Names ManuallyOne of the columns in the sequence

178 Understanding Your Agilent ChemStation8 AutomationPostsequence OperationPostsequence Operation You can specify what happens after a sequence has f

Understanding Your Agilent ChemStation 179Automation8Automatic RecalibrationAutomatic Recalibration Calibration is often done after a change in operat

18 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareAll ChemStation logbooks report system messages i

180 Understanding Your Agilent ChemStation8 AutomationSpecifying RecalibrationsSpecifying RecalibrationsThe recalibration parameters for the sequence

Understanding Your Agilent ChemStation 181Automation8Specifying RecalibrationsThe table shows the columns in the sequence table that contain the recal

182 Understanding Your Agilent ChemStation8 AutomationSpecifying RecalibrationsDelta%The delta% calculation allows you to compare response factors fro

Understanding Your Agilent ChemStation 183Automation8Types of SequencesTypes of SequencesThe following are types of sequences:• explicit calibration s

184 Understanding Your Agilent ChemStation8 AutomationExplicit Calibration SequencesExplicit Calibration Sequences This type of sequence recalibrates

Understanding Your Agilent ChemStation 185Automation8Cyclic Single-Level Calibration SequencesCyclic Single-Level Calibration Sequences This type of s

186 Understanding Your Agilent ChemStation8 AutomationCyclic Multiple-Level Calibration SequencesCyclic Multiple-Level Calibration Sequences This type

Understanding Your Agilent ChemStation 187Automation8Cyclic Multiple-Level Calibration SequencesMethod A Analysis Order This section describes the ana

188 Understanding Your Agilent ChemStation8 AutomationCyclic Multiple-Level Calibration SequencesMethod B Analysis OrderThis section describes the ana

Understanding Your Agilent ChemStation 189Automation8Cyclic Multiple-Level Calibration SequencesNote that the results shown in Table 17 on page 187and

Understanding Your Agilent ChemStation 19Agilent ChemStation Features1About the ChemStation Softwareallow rapid access to instrument parameters and an

190 Understanding Your Agilent ChemStation8 AutomationExplicit and Cyclic Calibrations TogetherExplicit and Cyclic Calibrations TogetherThis type of s

Understanding Your Agilent ChemStation 191Automation8Explicit and Cyclic Calibrations Together• The first calibration line is for the same level but a

192 Understanding Your Agilent ChemStation8 AutomationCyclic Calibration Sequences with BracketingCyclic Calibration Sequences with BracketingIn a cyc

Understanding Your Agilent ChemStation 193Automation8Cyclic Calibration Sequences with BracketingFigure 41 BracketingBracketed Sequence Operation • Th

194 Understanding Your Agilent ChemStation8 AutomationCyclic Calibration Sequences with BracketingSequence TableThe sequence table of Brack.M (next pa

Understanding Your Agilent ChemStation 195Automation8Cyclic Calibration Sequences with BracketingBracketed Sequence Analysis Order

196 Understanding Your Agilent ChemStation8 AutomationCyclic Recalibration Sequences with Multiple Vials Containing the Same Dilution of a StandardCyc

Understanding Your Agilent ChemStation 197Automation8Cyclic Recalibration Sequences with Multiple Vials Containing the Same Dilution of a StandardThe

198 Understanding Your Agilent ChemStation8 AutomationCyclic Recalibration Sequences with Multiple Vials Containing the Same Dilution of a StandardCyc

Understanding Your Agilent ChemStation 199Automation8Cyclic Recalibration Sequences with Multiple Vials Containing the Same Dilution of a StandardThe

Understanding Your Agilent ChemStationNotices© Agilent Technologies, Inc. 2004, 2005-2008No part of this manual may be reproduced in any form or by an

20 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareDuring an analysis, the complete functionality of

200 Understanding Your Agilent ChemStation8 AutomationCyclic Recalibration Sequences with Multiple Vials Containing the Same Dilution of a Standard

201Understanding Your Agilent ChemStationAgilent Technologies9Data Review, Reprocessing and Batch ReviewNavigation Table in Data Analysis 202Navigatio

202 Understanding Your Agilent ChemStation9 Data Review, Reprocessing and Batch ReviewNavigation Table in Data AnalysisNavigation Table in Data Analys

Understanding Your Agilent ChemStation 203Data Review, Reprocessing and Batch Review9Navigation Table in Data AnalysisThe Navigation table includes st

204 Understanding Your Agilent ChemStation9 Data Review, Reprocessing and Batch ReviewNavigation Table in Data AnalysisData Review ToolsetThe review f

Understanding Your Agilent ChemStation 205Data Review, Reprocessing and Batch Review9Navigation Table in Data Analysisis visible in the Status Bar; th

206 Understanding Your Agilent ChemStation9 Data Review, Reprocessing and Batch ReviewNavigation Table in Data Analysis• sequence-related batch (*.b)

Understanding Your Agilent ChemStation 207Data Review, Reprocessing and Batch Review9What is Batch Review?What is Batch Review?Batch review is a capab

208 Understanding Your Agilent ChemStation9 Data Review, Reprocessing and Batch ReviewEnabling Batch Review Functionality with ChemStation OpenLAB Opt

Understanding Your Agilent ChemStation 209Data Review, Reprocessing and Batch Review9Batch ConfigurationBatch ConfigurationA batch is a user-selected

Understanding Your Agilent ChemStation 21Agilent ChemStation Features1About the ChemStation Software• user-defined annotations may be interactively ad

210 Understanding Your Agilent ChemStation9 Data Review, Reprocessing and Batch ReviewBatch Configuration• the compound list contains all compounds fo

Understanding Your Agilent ChemStation 211Data Review, Reprocessing and Batch Review9Review FunctionsReview FunctionsData files can be displayed in on

212 Understanding Your Agilent ChemStation9 Data Review, Reprocessing and Batch ReviewBatch ReportingBatch ReportingThe user-configurable “Batch Table

213Understanding Your Agilent ChemStationAgilent Technologies10Using the ChemStation ReportsWhat is a Report? 214Reporting Results 215Uncalibrated Rep

214 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsWhat is a Report?What is a Report? A report can comprise quantitative and qu

Understanding Your Agilent ChemStation 215Using the ChemStation Reports10Reporting ResultsReporting ResultsTwo types of reports are available:• an unc

216 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsReporting Resultscalibration and unknown samples must be known accurately. T

Understanding Your Agilent ChemStation 217Using the ChemStation Reports10Quantitative ResultsQuantitative ResultsThe report type is identified by the

218 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsQuantitative ResultsISTD% produces a report of the relative amount of each s

Understanding Your Agilent ChemStation 219Using the ChemStation Reports10Reporting Custom Field ValuesReporting Custom Field ValuesThe values of the c

22 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareData Analysis — Data Review, Data Reprocess and B

220 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsReport StylesReport Styles The following report styles are available:You cho

Understanding Your Agilent ChemStation 221Using the ChemStation Reports10Report StylesFor uncalibrated methods, the report parameters include the peak

222 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsReport StylesAdding a Customized Report to Report StylesYou can add a custom

Understanding Your Agilent ChemStation 223Using the ChemStation Reports10Other Report Style ParametersOther Report Style ParametersSummed Peaks TableT

224 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsReport DestinationReport Destination The report can be sent to either:• Scre

Understanding Your Agilent ChemStation 225Using the ChemStation Reports10Report Destination.CSV The report is in Comma Separated Values (CSV) format.

226 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsSequence Summary ReportingSequence Summary ReportingOverviewThe ChemStation

Understanding Your Agilent ChemStation 227Using the ChemStation Reports10Sequence Summary ReportingOne Page HeaderThe GLP template prints GLP in large

228 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsSequence Summary Reportingprints the statistical trends of the analyses as g

Understanding Your Agilent ChemStation 229Using the ChemStation Reports10Sequence Summary ReportingSequence OutputIn the Sequence output dialog box yo

Understanding Your Agilent ChemStation 23Agilent ChemStation Features1About the ChemStation SoftwareData Analysis — Specialized ReportingAdvanced repo

230 Understanding Your Agilent ChemStation10 Using the ChemStation ReportsSequence Summary Reporting

231Understanding Your Agilent ChemStationAgilent Technologies11Evaluating System SuitabilityNoise Determination 235Noise Calculation Using Six Times t

232 Understanding Your Agilent ChemStation11 Evaluating System SuitabilitySequence Summary ReportingRegression Coefficient 253Standard Deviation (S) 2

Understanding Your Agilent ChemStation 233Evaluating System Suitability11Sequence Summary Reporting• peak width at half height, • peak symmetry,• peak

234 Understanding Your Agilent ChemStation11 Evaluating System SuitabilitySequence Summary Reporting• column/capillary details,• analytical method,• s

Understanding Your Agilent ChemStation 235Evaluating System Suitability11Noise DeterminationNoise DeterminationNoise can be determined from the data p

236 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityNoise DeterminationStd is the standard deviation of the linear regression of

Understanding Your Agilent ChemStation 237Evaluating System Suitability11Noise DeterminationNoise Calculation by the ASTM MethodFigure 44 Noise Determ

238 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityNoise DeterminationVery-short-term noise is determined for a selected time r

Understanding Your Agilent ChemStation 239Evaluating System Suitability11Noise DeterminationSignal-to-noise calculationFor the signal-to-noise calcula

24 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareThe Extended Performance style adds plots of each

240 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityCalculation of Peak SymmetryCalculation of Peak SymmetryThe ChemStation does

Understanding Your Agilent ChemStation 241Evaluating System Suitability11Calculation of Peak SymmetryFigure 45 Calculation of the Peak Symmetry Factor

242 Understanding Your Agilent ChemStation11 Evaluating System SuitabilitySystem Suitability Formulae and CalculationsSystem Suitability Formulae and

Understanding Your Agilent ChemStation 243Evaluating System Suitability11General DefinitionsGeneral DefinitionsVoid Volumewhere:d = diameter of column

244 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityPerformance Test DefinitionsPerformance Test DefinitionsStatistical Momentsw

Understanding Your Agilent ChemStation 245Evaluating System Suitability11Performance Test DefinitionsStatistical Moments, Skew and ExcessStatistical m

246 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityPerformance Test DefinitionsTrue Peak Width Wx [min]Capacity Factor (USP), C

Understanding Your Agilent ChemStation 247Evaluating System Suitability11Performance Test DefinitionsFigure 46 Performance ParametersNumber of Theoret

248 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityPerformance Test Definitions5 Sigma method:where:W4.4 = peak width at 4.4% o

Understanding Your Agilent ChemStation 249Evaluating System Suitability11Performance Test DefinitionsResolution (USP, ASTM) R(Pertaining to peaks a an

Understanding Your Agilent ChemStation 25Agilent ChemStation Features1About the ChemStation Softwareorganize them in information sections and graphica

250 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityDefinitions for ReproducibilityDefinitions for ReproducibilityFor the statis

Understanding Your Agilent ChemStation 251Evaluating System Suitability11Definitions for ReproducibilityThe sample standard deviation S differs in two

252 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityDefinitions for ReproducibilityConfidence Interval CIThe confidence interval

Understanding Your Agilent ChemStation 253Evaluating System Suitability11Definitions for ReproducibilityRegression AnalysisLetN = number of discrete o

254 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityDefinitions for ReproducibilityStandard Deviation (S)

Understanding Your Agilent ChemStation 255Evaluating System Suitability11Internally Stored Double Precision Number AccessInternally Stored Double Prec

256 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityInternally Stored Double Precision Number Access• Area• Height• Width (integ

Understanding Your Agilent ChemStation 257Evaluating System Suitability11Internally Stored Double Precision Number Access• Resolution – 5-Sigma (Exten

258 Understanding Your Agilent ChemStation11 Evaluating System SuitabilityInternally Stored Double Precision Number Access

259Understanding Your Agilent ChemStationAgilent Technologies12System VerificationVerification and Diagnosis Views 260System Verification 260The GLPsa

26 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareThe ChemStation includes commands and functions t

260 Understanding Your Agilent ChemStation12 System VerificationVerification and Diagnosis ViewsVerification and Diagnosis ViewsIf supported by the co

Understanding Your Agilent ChemStation 261System Verification12Verification and Diagnosis ViewsVerification test results are saved in binary format to

262 Understanding Your Agilent ChemStation12 System VerificationVerification and Diagnosis Views

Understanding Your Agilent ChemStation 263System Verification12The GLPsave RegisterThe GLPsave RegisterThe GLPsave register is saved at the end of eac

264 Understanding Your Agilent ChemStation12 System VerificationThe GLPsave Register• reload and reprint the data analysis part of the method used at

Understanding Your Agilent ChemStation 265System Verification12DAD Test FunctionDAD Test FunctionDetector tests can be used as a step in the routine s

266 Understanding Your Agilent ChemStationIndexIndexAabortsequence 170absoluteresponse factor 116retention time 130retention time 132accuracyanalysis

Understanding Your Agilent ChemStation 267Indexconfidence interval 252configuration 15control chart reports 25control limits 210corrected retention ti

268 Understanding Your Agilent ChemStationIndexmanuals 32method information 43method fileinstrument parameters 51methodcreate 48directory 51edit 49GLP

Understanding Your Agilent ChemStation 269Indexaverage 181complete 157interval 181partial 157retention time 157unidentified peaks 157what is? 157why 1

Understanding Your Agilent ChemStation 27Agilent ChemStation Features1About the ChemStation Softwaretable can be configured by the user. The user can

270 Understanding Your Agilent ChemStationIndexsignal-to-noise calculation 239single reference peaks 134single-level calibrationcyclic sequences 185sk

© Agilent Technologies 2004, 2005-2008Printed in Germany 07/08*G2070-91125**G2070-91125*G2070-91125www.agilent.comAgilent TechnologiesIn This BookThis

28 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareGood Laboratory PracticeThe ChemStation is develo

Understanding Your Agilent ChemStation 29Agilent ChemStation Features1About the ChemStation SoftwareThe ChemStation may be configured for restricted a

Understanding Your Agilent ChemStation 3In This Guide...In This Guide...This guide describes various concepts of the Agilent ChemStation. It is intend

30 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesAbout the ChemStation SoftwareAll reports have time stamps and traceable page n

Understanding Your Agilent ChemStation 31Agilent ChemStation Features1Instrument ControlInstrument ControlThe instrument control capability of the Che

32 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesDocumentationDocumentationThe documentation set contains specific sections on:

Understanding Your Agilent ChemStation 33Agilent ChemStation Features1DocumentationUnderstanding the PrinciplesThe Understanding Your ChemStation manu

34 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesThe ChemStation Directory StructureThe ChemStation Directory StructureThe follo

Understanding Your Agilent ChemStation 35Agilent ChemStation Features1The ChemStation Directory StructureFigure 1 ChemStation Directory Structure8=:B(

36 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesThe ChemStation Directory StructureTable 3 ChemStation subdirectoriesDirectory

Understanding Your Agilent ChemStation 37Agilent ChemStation Features1The ChemStation Directory StructureSEQUENCE Comprises the default path for seque

38 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesNavigation PaneNavigation PaneA Navigation Pane, available on the left side of

Understanding Your Agilent ChemStation 39Agilent ChemStation Features1Navigation PaneVerification (LC and LC/MS) Verification view-specific shortcutsD

4 Understanding Your Agilent ChemStationIn This Guide...7 CalibrationThis chapter describes Calibration in the ChemStation.8AutomationThis chapter des

40 Understanding Your Agilent ChemStation1 Agilent ChemStation FeaturesNavigation Pane

41Understanding Your Agilent ChemStationAgilent Technologies2MethodsWhat is a Method? 42Parts of a Method 43Method Information 43Instrument Control 43

42 Understanding Your Agilent ChemStation2 MethodsWhat is a Method?What is a Method?A method comprises all the parameters for acquisition and data ana

Understanding Your Agilent ChemStation 43Methods2Parts of a MethodParts of a MethodA method is identified by a name of up to forty alphanumeric charac

44 Understanding Your Agilent ChemStation2 MethodsParts of a MethodSignal DetailsDefines signals and their properties used for data evaluation.Integra

Understanding Your Agilent ChemStation 45Methods2Parts of a MethodRun-Time Checklist Defines which parts of the method are executed when the method is

46 Understanding Your Agilent ChemStation2 MethodsStatus of MethodsStatus of MethodsA method can exist in two states: as a stored method, or as the cu

Understanding Your Agilent ChemStation 47Methods2Status of Methodsselected. Changes to this method (for example, timed integrations events) are specif

48 Understanding Your Agilent ChemStation2 MethodsCreating MethodsCreating Methods Creating a new method always means modifying the current method and

Understanding Your Agilent ChemStation 49Methods2Editing MethodsEditing MethodsYou can edit an existing method using the Edit Entire Method item of th

Understanding Your Agilent ChemStation 5ContentsContents1 Agilent ChemStation Features 9General Description 11ChemStation Hardware 14About the ChemSta

50 Understanding Your Agilent ChemStation2 MethodsEditing Methods• Instrument Control depends on the configuration, and can comprise, for example:• ov

Understanding Your Agilent ChemStation 51Methods2Method Directory StructureMethod Directory StructureA method comprises a group of files stored in the

52 Understanding Your Agilent ChemStation2 MethodsWhat Happens When a Method is Run?What Happens When a Method is Run?The run-time checklist dialog bo

Understanding Your Agilent ChemStation 53Methods2What Happens When a Method is Run?Figure 3 Method OperationPre-run Command or Macro (Run Time Checkli

54 Understanding Your Agilent ChemStation2 MethodsWhat Happens When a Method is Run?Data Analysis (Run Time Checklist)When the stop-time has elapsed,

Understanding Your Agilent ChemStation 55Methods2What Happens When a Method is Run?Peak Purity Checking (ChemStations for LC 3D, CE, CE/MS and LC/MS S

56 Understanding Your Agilent ChemStation2 MethodsWhat Happens When a Method is Run?These data are saved only if the Save GLP Data feature is activate

Understanding Your Agilent ChemStation 57Methods2Method Operation SummaryMethod Operation SummaryThe following list shows the flow of the method opera

58 Understanding Your Agilent ChemStation2 MethodsMethod Operation Summary

59Understanding Your Agilent ChemStationAgilent Technologies3Data AcquisitionWhat is Data Acquisition? 60Data Files 61Online Monitors 63Online Signal

6 Understanding Your Agilent ChemStationContentsPrinciple of Operation 79Peak Recognition 80Baseline Allocation 89Peak Area Measurement 102Integration

60 Understanding Your Agilent ChemStation3 Data AcquisitionWhat is Data Acquisition?What is Data Acquisition?During data acquisition, all signals acqu

Understanding Your Agilent ChemStation 61Data Acquisition3Data FilesData Files A data file comprises a group of files, by default stored in the DATA d

62 Understanding Your Agilent ChemStation3 Data AcquisitionData FilesThe method can be stored with the result files (run.m). In such cases the method

Understanding Your Agilent ChemStation 63Data Acquisition3Online MonitorsOnline Monitors There are two types of online monitors, the online signal mon

64 Understanding Your Agilent ChemStation3 Data AcquisitionLogbookLogbook The logbook displays messages that are generated by the analytical system. T

Understanding Your Agilent ChemStation 65Data Acquisition3Status InformationStatus InformationChemStation StatusThe ChemStation Status window shows a

66 Understanding Your Agilent ChemStation3 Data AcquisitionStatus InformationSystem DiagramIf supported by the configured analytical instruments (for

67Understanding Your Agilent ChemStationAgilent Technologies4IntegrationWhat is Integration? 69What Does Integration Do? 70The ChemStation Integrator

68 Understanding Your Agilent ChemStation4 IntegrationStatus InformationPeak Valley Ratio 92Tangent Skimming 94Unassigned Peaks 99Peak Separation Code

Understanding Your Agilent ChemStation 69Integration4What is Integration?What is Integration?Integration locates the peaks in a signal and calculates

Understanding Your Agilent ChemStation 7Contents8 Automation 159What is Automation? 161What is a Sequence/Sequence Template? 162Preferences - Sequence

70 Understanding Your Agilent ChemStation4 IntegrationWhat Does Integration Do?What Does Integration Do? When a signal is integrated, the software:• i

Understanding Your Agilent ChemStation 71Integration4The ChemStation Integrator AlgorithmsThe ChemStation Integrator AlgorithmsThe ChemStation integra

72 Understanding Your Agilent ChemStation4 IntegrationThe ChemStation Integrator Algorithms• the ability of baseline correction parameters (non signal

Understanding Your Agilent ChemStation 73Integration4OverviewOverviewTo integrate a chromatogram/electropherogram the integrator:1 defines the initial

74 Understanding Your Agilent ChemStation4 IntegrationOverviewBefore the integrator can integrate peaks, it must establish a baseline point. At the be

Understanding Your Agilent ChemStation 75Integration4OverviewAllocating the BaselineThe integrator allocates the chromatographic/electropherographic b

76 Understanding Your Agilent ChemStation4 IntegrationOverviewIdentifying the Cardinal Points of a PeakThe integrator determines that a peak may be st

Understanding Your Agilent ChemStation 77Integration4Definition of TermsDefinition of TermsCardinal PointsFigure 5 Cardinal pointsCardinal points are

78 Understanding Your Agilent ChemStation4 IntegrationDefinition of TermsShoulder (front, rear)Shoulders occur when two peaks elute so close together

Understanding Your Agilent ChemStation 79Integration4Principle of OperationPrinciple of OperationFigure 6 Integrator Flow Diagram>c^i^VaeVgVbZiZgh

8 Understanding Your Agilent ChemStationContentsReporting Custom Field Values 219Report Styles 220Other Report Style Parameters 223Report Destination

80 Understanding Your Agilent ChemStation4 IntegrationPeak RecognitionPeak RecognitionThe integrator uses several tools to recognize and characterize

Understanding Your Agilent ChemStation 81Integration4Peak RecognitionIn Figure Peak Width Calculation, the total area, A, is the sum of the areas a1,

82 Understanding Your Agilent ChemStation4 IntegrationPeak RecognitionTo obtain good performance from the recognition filters, the peak width must be

Understanding Your Agilent ChemStation 83Integration4Peak RecognitionWhen data is bunched, the data points are bunched as two raised to the bunching p

84 Understanding Your Agilent ChemStation4 IntegrationPeak RecognitionFigure 8 Peak recognitionThe criteria are as follows:t1 Upslope counter is great

Understanding Your Agilent ChemStation 85Integration4Peak Recognitiont6 • Peak valley found and upslope counter is greater than or equal to 2 or• Down

86 Understanding Your Agilent ChemStation4 IntegrationPeak RecognitionPeak EndIn Table 10 on page 86the expected peak width determines which filter’s

Understanding Your Agilent ChemStation 87Integration4Peak RecognitionFigure 9 Merged PeaksThe integrator processes merged peaks in the following way:1

88 Understanding Your Agilent ChemStation4 IntegrationPeak RecognitionFigure 10 Peak ShouldersShoulders are detected from the curvature of the peak as

Understanding Your Agilent ChemStation 89Integration4Baseline AllocationBaseline AllocationAfter any peak cluster is complete, and the baseline is fou

9Understanding Your Agilent ChemStationAgilent Technologies1Agilent ChemStation FeaturesGeneral Description 11Additional Instrument Modules 12Addition

90 Understanding Your Agilent ChemStation4 IntegrationBaseline AllocationThe Start of the BaselineIf no baseline is found at the start of the run, the

Understanding Your Agilent ChemStation 91Integration4Baseline AllocationBaseline Penetration A penetration occurs when the signal drops below the cons

92 Understanding Your Agilent ChemStation4 IntegrationBaseline AllocationFigure 13 Standard baseline tracking and baseline tracking (no penetration)Ad

Understanding Your Agilent ChemStation 93Integration4Baseline AllocationFigure 14 Peak Valley RatioThe peak valley ratio is calculated using the follo

94 Understanding Your Agilent ChemStation4 IntegrationBaseline AllocationTangent SkimmingTangent skimming is a form of baseline constructed for peaks

Understanding Your Agilent ChemStation 95Integration4Baseline Allocationpeaks after the first child peak are separated by drop lines, beginning at the

96 Understanding Your Agilent ChemStation4 IntegrationBaseline AllocationStandard SkimsThe appropriate calculation is chosen for a particular applicat

Understanding Your Agilent ChemStation 97Integration4Baseline AllocationYou can disable exponential skimming throughout the run by setting the value o

98 Understanding Your Agilent ChemStation4 IntegrationBaseline AllocationThe exponential model is fitted through the part of the tail of the parent pe

Understanding Your Agilent ChemStation 99Integration4Baseline AllocationThe valley height ratio takes the same value for both front peak skimming and