Agilent Technologies All Troubleshooting Guide download pdf (Page 102)

Lab Exercise: Quantification

Building a Calibration Table

You have already loaded and integrated the lowest level standard. The first step

toward building your calibration table will be to select the calibration settings.

1) In the Calibration menu, select Calibration Settings...

2) Fill in the Title of the Calibration Table, e.g., Class.

3) You will Use Sample Data From Data File.

4) Type in µg/mL for Amount Units.

5) The Default RT Windows should be set at 5%.

6) Select No for Calculate Uncalibrated Peaks.

7) If you do not understand any of these entries, read the online help. OK this

dialog box.

8) Under the Calibration menu, select New Calibration Table… or, select the

New Calibration Table from current chromatogram tool.

9) Select Automatic Setup Level 1. You may select a default amount for all

peaks or fill in the amount after entering the calibration table. For your

current purposes, leave the Default Amount as 0.00. Do not check the box to

Calculate Signals Separately because we will be using qualifiers. OK the

dialog box.

10) The calibration table now appears. You will be calibrating the last two of four

major chromatographic peaks. Locate the third major chromatographic peak

at approximately 1.9 minutes (for a mobile phase of acetonitrile). Use the

signal that provides the most area counts.

11) Click in the Compound column, and type in Biphenyl; selecting the signal

that gives you the most area counts. In the Amt[µg/ml] column, type in 0.8.

Scroll to the right and make this a reference peak. If you do not see the

reference peak as an option, make certain that you are currently in the

Overview portion of the calibration table. This selection can be found in the

pull down box in the middle of the window.

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Agilent Technologies All Troubleshooting Guide Page 102