Agilent Technologies All Troubleshooting Guide Page 55
- Page / 211
- Table of contents
- BOOKMARKS
Rated. / 5. Based on customer reviews
Lab Exercise: Spectral Libraries
Library Searching
47
Library Searching
In this section you will test your library with the same data file. Later we will use
the library with new files in an automated fashion. Check that the library file you
want to search from is currently loaded (look in the Library tool bar).
Normally, if you were going to perform a library search, you would have to open
the library first. Open Library is found under the Spectra, Library menus. You
could also use the Load a Library File tool.
1) From the Spectra menu, select Library, Edit Search Template. You will
perform the search within a retention time window. Select Left Window (%)
and type in 5. Give the Right Window (%) a value of 5 as well. You have
now defined a time range in the library within which spectra are searched, +
or - 5% of the retention time of the unknown.
2) Give the threshold a value of 2 mAU. Low absorbing portions of the spectra
will be removed and not used in the match calculation improving the
reliability of the match.
3) You do not need to put in a shift. This might be useful if your data and the
library data were acquired on different instruments. OK the template.
4) Under the Spectra menu, choose Select Peak Apex Spectrum or use the
Peak apex spectrum tool. Select the apex spectrum from the first
chromatographic peak. Under the Spectra menu, select Library, Search
Spectrum or use the Search the Spectrum in Current Library tool.
5) The Library Search Results are displayed in the lower right hand side of the
screen. Note that the best match is Dimethylphthalate. If other matches are
listed, they were found within the specified retention time window. Did you
get a perfect match of 1000?
6) Examine the Spectral Difference window. Because you have a perfect
match, the difference should be zero.
7) Notice also that you have the unknown (target) and the library spectrum
displayed in the window on the left.
8) Perform library searches on the other peaks in the chromatogram. Do not
delete this library file. You will use it later for automated library searching
later.
- Agilent 1100/1200 HPLC 1
- ChemStation Operation 1
- Course Number H4033A 3
- Laboratory Manual 3
- Table Of Contents 5
- ChemStation 9
- In this Laboratory You Will: 10
- Configuration Editor 13
- Scheduler 14
- Windows XP) 16
- Cleaning up Temporary Files 16
- Repairing the Disk 16
- Disk Defragmentation 17
- Workstation) 18
- (Windows 2000 or XP) 21
- Windows Explorer 21
- Wallpaper and Screen Savers 22
- Clipboard 23
- Preparation 27
- Preparing the HPLC 28
- Practice Entering Gradients 32
- OK the dialog box 34
- Snapshot 35
- Graphics - Signal Options 39
- Graphics - Annotation 41
- Window Functions 43
- Peak Purity 44
- Displaying Spectra 47
- Isoabsorbance Plot 48
- 3D Plot 50
- Building a Library 53
- Library Searching 55
- The Agilent 1200 Flow Path 59
- Diagnosis View 61
- Diagnosis Instrument Panel 62
- Instrument Tests 64
- Routine Maintenance 67
- Agilent LC Diagnostic Tool 73
- Diagnosing Common Problems 74
- Instrument Status Report 77
- Lab Exercise: Integration 81
- Integration Preparation 83
- Auto Integration 84
- Adding Timed Events 87
- Using Manual Events 89
- Data Acquisition-Standards 97
- Integration 99
- Setting up Signal Details 101
- Building a Calibration Table 102
- Calibration Standards 104
- Building a Sequence 109
- Sequence Summary Reports 112
- Starting the Sequence 113
- Pausing a Sequence 114
- Post Sequence 115
- Reprocessing 117
- Reprocess with DA Methods 125
- Batch Review 129
- Adding a Report Header 133
- Automated Peak Purity 137
- Preparations 143
- Building a Customized Report 144
- Adding a General Section 145
- Inserting Tables 146
- Adding a Calibration Curve 147
- Finishing Up 148
- Lab Exercise: Commands 149
- In this LaboratoryYou Will: 150
- Commands 152
- Registers 153
- Objects 154
- Lab Exercise: Macro Writing 161
- Write Your Own Macro 165
- Possible Answer 166
- Agilent ChemStation 168
- Getting Started with New 168
- ChemStation Workflow 168
- In This Guide… 170
- 4 Data Organization 171
- Contents 172
- 6 Data Organization 173
- ChemStation Data Structure 174
- ChemStation prior to B.02.01 175
- ChemStation B.02.01/B.03.01 176
- 12 Data Organization 179
- 1 ChemStation Data Structure 179
- Data Acquisition 180
- 18 Data Organization 185
- 2 Data Acquisition 185
- Data Analysis 186
- 22 Data Organization 189
- 3 Data Analysis 189
- B.02.01 and higher 189
- Data Analysis: Data Review 190
- Keep “Current Method” 191
- Load “Sequence Method” 192
- Update master method feature 196
- Update Sequence Method 197
- Master method — 197
- Data Organization 33 200
- Data Analysis 3 200
- 34 Data Organization 201
- Agilent Technologies 202
- 42 Data Organization 209
- *G2170-90041* 211
Related products and manuals for Measuring, testing & control Agilent Technologies All
(34 pages)© 2020, manymanuals.com. All rights reserved. | 4.314 s |
Manymanuals.com
Manymanuals.de
Manymanuals.fr
Manymanuals.it
Manymanuals.pl
Manymanuals.cz
Manymanuals.es
Manymanuals-pt.com
Comments to this Manuals