Agilent Technologies All Troubleshooting Guide download pdf (Page 85)

Lab Exercise: Integration

Auto Integration

5) Using the Events Table drop box, select signal DAD1 B Specific.

6) To Auto Integrate, select the Integration tool then the Auto Integrate tool

or select the Integration menu, then Auto Integrate. When asked if you

want to save the changed events table, reply No.

7) View the events and chromatogram that were created for Signal B.

8) Now see what happens when you add the recommended For All Signals

settings:

Tangent Skim Mode: New Exponential

Tail Peak Skim Height Ratio: 3.00

Front Peak Skim Height Ratio: 6.00

Skim Valley Ratio: 20.00

Baseline Correction: Advanced

Peak to Valley Ratio 500.00

Note: Auto Integrate does not set the For All Signals Integration Events. You

must set these manually.

10) Use Auto Integrate again. Again, do not save the changed events table.

Print the chromatogram to notice any differences. You may want to print

this chromatogram for comparison and record the event settings below.

Did you see any improvement?

Initial Slope Sensitivity__________

Initial Peak Width __________

Initial Area Reject __________

Initial Height Reject __________

Initial Shoulders __________

1 2 ... 80 81 82 83 84 85 86 87 88 89 90 ... 210 211

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Agilent Technologies All Troubleshooting Guide Page 85