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Lab Exercise: Overview and Diagnostics for the Agilent-SL Series
Agilent LC Diagnostic Tool
65
Agilent LC Diagnostic Tool
The Agilent 1200 Series HPLC- SL modules are shipped with a tool to help you:
1) troubleshoot problems such as high pressure errors, 2) perform diagnostic tests,
such as the pressure test, 3) perform calibrations, 4) provide you with a means to
determine when instrument maintenance should be performed, Early Maintenance
Feedback (EMF), and 5) provide instrument status reports. In this section, you
will explore the software to become acquainted with its layout and function. In
later sections, you will perform diagnostic tests and maintenance from this
software.
Early Maintenance Feedback (EMF)
The Early Maintenance Feedback system allows the user to schedule preventive
maintenance based upon instrument usage. For instance, seals may be replaced
based upon seal wear and the rotor seal may be replaced based upon the number
of valve switches. The analyst inputs usage limits based upon previous
maintenance experience. For instance, if the rotor seal previously failed after
20,000 injections, a limit of 18,000 valve switches might be inserted as the EMF
valve switch limit. When 18,000 injections have been made, the EMF indicator
found in the Method and Run Control view would change from a green check
mark to a yellow question mark indicating that one of the EMF limits had been
reached. In this part of the laboratory, you will practice inserting EMF limits and
observe the EMF indicator.
1) Go to the Diagnosis view. Double click on the Agilent LC Diagnostic tool
found in the Explorer and then Start the tool.
2) Access the Tools button.
3) Highlight the EMF Editor, then Start.
4) In the Instrument/Module Selection tree, select the pump.
5) Hightlight the Seal Wear line. To test the EMF system, set the seal wear
limit 2 units above the actual seal wear then Set Limit. Note: The seal wear
counter increases as a function of both flow rate and pressure. If your
system does not have a column installed, the pressure will be quite low. In
- Agilent 1100/1200 HPLC 1
- ChemStation Operation 1
- Course Number H4033A 3
- Laboratory Manual 3
- Table Of Contents 5
- ChemStation 9
- In this Laboratory You Will: 10
- Configuration Editor 13
- Scheduler 14
- Windows XP) 16
- Cleaning up Temporary Files 16
- Repairing the Disk 16
- Disk Defragmentation 17
- Workstation) 18
- (Windows 2000 or XP) 21
- Windows Explorer 21
- Wallpaper and Screen Savers 22
- Clipboard 23
- Preparation 27
- Preparing the HPLC 28
- Practice Entering Gradients 32
- OK the dialog box 34
- Snapshot 35
- Graphics - Signal Options 39
- Graphics - Annotation 41
- Window Functions 43
- Peak Purity 44
- Displaying Spectra 47
- Isoabsorbance Plot 48
- 3D Plot 50
- Building a Library 53
- Library Searching 55
- The Agilent 1200 Flow Path 59
- Diagnosis View 61
- Diagnosis Instrument Panel 62
- Instrument Tests 64
- Routine Maintenance 67
- Agilent LC Diagnostic Tool 73
- Diagnosing Common Problems 74
- Instrument Status Report 77
- Lab Exercise: Integration 81
- Integration Preparation 83
- Auto Integration 84
- Adding Timed Events 87
- Using Manual Events 89
- Data Acquisition-Standards 97
- Integration 99
- Setting up Signal Details 101
- Building a Calibration Table 102
- Calibration Standards 104
- Building a Sequence 109
- Sequence Summary Reports 112
- Starting the Sequence 113
- Pausing a Sequence 114
- Post Sequence 115
- Reprocessing 117
- Reprocess with DA Methods 125
- Batch Review 129
- Adding a Report Header 133
- Automated Peak Purity 137
- Preparations 143
- Building a Customized Report 144
- Adding a General Section 145
- Inserting Tables 146
- Adding a Calibration Curve 147
- Finishing Up 148
- Lab Exercise: Commands 149
- In this LaboratoryYou Will: 150
- Commands 152
- Registers 153
- Objects 154
- Lab Exercise: Macro Writing 161
- Write Your Own Macro 165
- Possible Answer 166
- Agilent ChemStation 168
- Getting Started with New 168
- ChemStation Workflow 168
- In This Guide… 170
- 4 Data Organization 171
- Contents 172
- 6 Data Organization 173
- ChemStation Data Structure 174
- ChemStation prior to B.02.01 175
- ChemStation B.02.01/B.03.01 176
- 12 Data Organization 179
- 1 ChemStation Data Structure 179
- Data Acquisition 180
- 18 Data Organization 185
- 2 Data Acquisition 185
- Data Analysis 186
- 22 Data Organization 189
- 3 Data Analysis 189
- B.02.01 and higher 189
- Data Analysis: Data Review 190
- Keep “Current Method” 191
- Load “Sequence Method” 192
- Update master method feature 196
- Update Sequence Method 197
- Master method — 197
- Data Organization 33 200
- Data Analysis 3 200
- 34 Data Organization 201
- Agilent Technologies 202
- 42 Data Organization 209
- *G2170-90041* 211
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