Agilent Technologies All Troubleshooting Guide download pdf (Page 47)

Lab Exercise: Qualitative Data Analysis

Displaying Spectra

1) With demo\demodad.d still loaded, use the signals drop box to select only one

signal for the window. If necessary, enlarge the chromatogram window by

grabbing the bottom of the window and dragging to view the peaks more

easily.

2) Under the Spectra menu, select Spectra Options or select the Spectral Task

tool, then the Edit Spectra Display and Processing Options tool.

3) Select the type of background subtraction best suited for this analysis in the

Reference tab. Try Automatic or select your own spectra. OK this dialog

box.

4) Go to the Spectra menu and choose Select Spectrum or click on the Select

Spectrum tool.

5) Position the mouse pointer on a peak of interest at the upslope of the

chromatographic peak and click the left mouse button. The upslope is half

way up the left side. Notice that the Reference Spectra and the Original

Spectrum are displayed in one window (on the right). The subtracted

spectrum is displayed in the other window (on the left).

6) Now overlay the background subtracted downslope spectrum. The downslope

spectrum is on the right-hand side of the peak. To do this hold the CTRL key

down while clicking the left mouse button.

What information can you obtain by comparing the upslope and downslope

spectra?

7) From the Spectra menu, choose any of the other select spectra items and

practice with them. Practice with the tools as well.

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Agilent Technologies All Troubleshooting Guide Page 47