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Table Of Contents
LAB EXERCISE: INTRODUCTION TO THE HPLC CHEMSTATION..... 1
IN THIS LABORATORY YOU WILL: ....................................................................... 2
ACCESSING THE HPLC CHEMSTATION................................................................ 3
C
ONFIGURATION EDITOR ..................................................................................... 5
S
CHEDULER ......................................................................................................... 6
MAINTAINING THE WINDOWS 2000 WORKSTATION ............................................ 8
Cleaning up Temporary Files ......................................................................... 8
Repairing the Disk .......................................................................................... 8
Disk Defragmentation..................................................................................... 9
Creating an Emergency Repair Disk .............................................................. 9
MAINTAINING THE WINDOWS XP WORKSTATION ............................................. 10
Cleaning up Temporary Files ....................................................................... 10
Repairing the Disk ........................................................................................ 10
Disk Defragmentation................................................................................... 11
Creating an Emergency Repair Disk ............................................................ 11
WINDOWS FEATURES (WINDOWS 2000 OR XP) ................................................. 13
Windows Explorer......................................................................................... 13
Wallpaper and Screen Savers ....................................................................... 14
Clipboard ...................................................................................................... 15
Closing a Program that is Not Operational ................................................. 15
LAB EXERCISE: ACQUISITION METHODS............................................. 17
IN THIS LABORATORY YOU WILL: ..................................................................... 18
PREPARATION .................................................................................................... 19
P
REPARING THE HPLC ...................................................................................... 20
PRIMING THE AGILENT 1100/1200 SOLVENT DELIVERY SYSTEM ...................... 21
C
REATING AN ACQUISITION METHOD................................................................ 23
P
RACTICE ENTERING GRADIENTS ...................................................................... 24
SNAPSHOT.......................................................................................................... 27
LAB EXERCISE: QUALITATIVE DATA ANALYSIS................................ 29
IN THIS LABORATORY YOU WILL: ..................................................................... 30
G
RAPHICS - SIGNAL OPTIONS............................................................................. 31
GRAPHICS - ANNOTATION.................................................................................. 33
W
INDOW FUNCTIONS......................................................................................... 35
PEAK PURITY ..................................................................................................... 36
A
UTOMATED PEAK PURITY................................................................................ 38
DISPLAYING SPECTRA........................................................................................ 39
I
SOABSORBANCE PLOT....................................................................................... 40
3D
PLOT ............................................................................................................ 42
- Agilent 1100/1200 HPLC 1
- ChemStation Operation 1
- Course Number H4033A 3
- Laboratory Manual 3
- Table Of Contents 5
- ChemStation 9
- In this Laboratory You Will: 10
- Configuration Editor 13
- Scheduler 14
- Windows XP) 16
- Cleaning up Temporary Files 16
- Repairing the Disk 16
- Disk Defragmentation 17
- Workstation) 18
- (Windows 2000 or XP) 21
- Windows Explorer 21
- Wallpaper and Screen Savers 22
- Clipboard 23
- Preparation 27
- Preparing the HPLC 28
- Practice Entering Gradients 32
- OK the dialog box 34
- Snapshot 35
- Graphics - Signal Options 39
- Graphics - Annotation 41
- Window Functions 43
- Peak Purity 44
- Displaying Spectra 47
- Isoabsorbance Plot 48
- 3D Plot 50
- Building a Library 53
- Library Searching 55
- The Agilent 1200 Flow Path 59
- Diagnosis View 61
- Diagnosis Instrument Panel 62
- Instrument Tests 64
- Routine Maintenance 67
- Agilent LC Diagnostic Tool 73
- Diagnosing Common Problems 74
- Instrument Status Report 77
- Lab Exercise: Integration 81
- Integration Preparation 83
- Auto Integration 84
- Adding Timed Events 87
- Using Manual Events 89
- Data Acquisition-Standards 97
- Integration 99
- Setting up Signal Details 101
- Building a Calibration Table 102
- Calibration Standards 104
- Building a Sequence 109
- Sequence Summary Reports 112
- Starting the Sequence 113
- Pausing a Sequence 114
- Post Sequence 115
- Reprocessing 117
- Reprocess with DA Methods 125
- Batch Review 129
- Adding a Report Header 133
- Automated Peak Purity 137
- Preparations 143
- Building a Customized Report 144
- Adding a General Section 145
- Inserting Tables 146
- Adding a Calibration Curve 147
- Finishing Up 148
- Lab Exercise: Commands 149
- In this LaboratoryYou Will: 150
- Commands 152
- Registers 153
- Objects 154
- Lab Exercise: Macro Writing 161
- Write Your Own Macro 165
- Possible Answer 166
- Agilent ChemStation 168
- Getting Started with New 168
- ChemStation Workflow 168
- In This Guide… 170
- 4 Data Organization 171
- Contents 172
- 6 Data Organization 173
- ChemStation Data Structure 174
- ChemStation prior to B.02.01 175
- ChemStation B.02.01/B.03.01 176
- 12 Data Organization 179
- 1 ChemStation Data Structure 179
- Data Acquisition 180
- 18 Data Organization 185
- 2 Data Acquisition 185
- Data Analysis 186
- 22 Data Organization 189
- 3 Data Analysis 189
- B.02.01 and higher 189
- Data Analysis: Data Review 190
- Keep “Current Method” 191
- Load “Sequence Method” 192
- Update master method feature 196
- Update Sequence Method 197
- Master method — 197
- Data Organization 33 200
- Data Analysis 3 200
- 34 Data Organization 201
- Agilent Technologies 202
- 42 Data Organization 209
- *G2170-90041* 211
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